Gomez Zumaquero, Juan Miguel
Coordinador Plataforma Genómica firstname.lastname@example.org
The Genomics shared research support platform provides mid- and high-level sequencing and genotyping services. This shared research support structure (ECAI) is accredited according to the ISO/IEC 17025:2005 standard.
The platform can provide any technical or methodological advising needed at any time. There are two modalities of services offered:
3130XL. 3130XL. 3130XL. Automatic DNA sequencing via capillary electrophoresis and using the BigDye Terminator kit from Applied Biosystems, obtaining readings of up to 1000 bases per reaction from plasmoids, PCR products, cosmids, phages, or BAC.
Analysis of DNA fragments marked with fluorochromes can be conducted using this equipment. It allows for combinations of fluorochromes (6-FAM, HEX, NED, ROX, VIC, PET, LIZ) for multiple genotyping.
The platform participates in external quality assessments of genetic studies for monogenic diabetes (ISO 17043) (EQA).
The Genomics shared research support structure offers massive sequencing and next-generation sequencing (NGS) services using the Ion-S5 platform from Thermo Fisher Scientific, which is based on semiconductor technology.
The system’s flexibility is owed to:
The Ion Torrent platform offers a wide range of applications. Applications of note are:
There are various technologies that allow for targeted resequencing or enrichment of libraries in targets/genes of interest that are compatible with the Ion Torrent platform:
– Cancer (cancer, BRCA1, BRCA2, lung, colon, etc.)
– Hereditary diseases (cardiovascular, haematology, autism, dermatology, deafness, etc.)
– Infectious diseases, apoptosis
– Human identification (forensic applications)
Pyrosequencing.. EThe PyroMark Q96 ID pyrosequencer is a fully integrated system that provides real-time information on sequences. It is one of the systems of reference for epigenetic research and genetic analysis. It also allows for the preparation of a single strand of DNA using the PyroMark Q96 ID Vacuum Workstation. Applications include:
The Sequencing and Genotyping Platform has equipment for independent use available, such as real-time PCR equipment.
In real-time PCR systems, in addition to quantitative PCR experiments, it is possible to carry out genotyping analyses using high-resolution melt curves, Taqman probes, HRM analysis, and more.
To request services from the Genomics shared research support platform, please complete the corresponding document and send it to email@example.com
The platform will send you an email to confirm receipt and begin the procedure for the requested service.