Proteomics

This ECAI is centralized in the Proteomics Unit of the University of Malaga (CPU, Servicios Centrales de Apoyo a la Investigación (SCAI) Central Services Research Support, UMA). It features a broad infrastructure and more than 7 years of experience

  • Objectives
  • Services
  • Members
  • Processing and proteomic analysis of basic and clinical research samples.
  • Advise users on all proteomic techniques.
  • Training in proteomics through courses and outreach and specialization seminars.
  • Continuous improvement of services offered.
  • Incorporation of new techniques and updating of established techniques.
  • Measurement of excitation and fluorescent emission spectra, fluorescence kinetics, fluorescence quenching studies, measurement of intracellular Ca2+, fluorescence polarization, determination of binding constants and ligand-protein dissociation, determination of rapid kinetics (in the range of 10-100 ms).
  • Identification of proteins from one- or two-dimensional gel electrophoresis blocks, as well as proteins in solution. Mass spectra/mass (MSn) with successive fragmentation of precursor ions, which allows for peptide sequencing. Metabolomic applications are also supported.
  • Analysis, separation and quantitative assessment of complex mixtures of proteins, peptides, amino acids, hormones and drugs by FPLC (fast protein liquid chromatography) and HPLC (high-performance liquid chromatography).
  • Determination of the exact molecular mass and post-translational modifications of proteins and peptides with a MALDI-TOF/TOF instrument, Q-Exactive Orbitrap and ion trap  ETD-AMAZON with electrospray ionization (ESI).
  • Protein profiling in clinical samples using fluorescence bidimensional differential electrophoresis (2D-DIGE) and MALDI-TOF/TOF.
  • Identification and characterization of biomarkers in clinical samples by separation with Protein-Chip array technology and mass SELDI-TOF spectrometry analysis.
  • Generation of profiling and protein maps in situ on tissue sections using MALDI-IMAGING technology in an UltrafleXtreme-MALDI-TOF/TOF mass spectrometer.
  • Daniel Torres Fernández
  • Francisco Javier Márquez Gómez
  • Mercedes Martín Rufián
Proteomics