General Research Services

This ECAI is located within all the hospitals, the Regional University Hospital in Malaga, the Civil Hospital, and the Virgen de la Victoria University Hospital. Its creation stems from the need to join the research efforts of various groups within the Clinical Departments that have established lines of research related to each other and common equipment needs. The research conducted in various research laboratories brings together the efforts of the groups engaged in research in different areas of knowledge.

Currently, the research laboratories offer complete facilities, some of which are in common use by the different groups and possible external users. In this common area, equipment is organized into different areas: flow cytometry, cell culture and immunochemistry, sample processing and sterilization.

These facilities are available to all IBIMA researchers. To this end, there is system for reserving equipment or services. Temporary access is provided, as well as consulting for researchers needing these facilities, to achieve the objectives of their research projects funded by different programs and who need to use the facilities on an ad hoc basis, following specific procedures.

  • Services
  • Members

Facilities and equipment are provided in optimal conditions, calibrated and periodically reviewed for their correct operation.

Cytometry Service

  • Immunophenotyping. Characterization of cell subpopulations, activation status, receptor expression and cell mediators
  • Feasibility studies.
  • Cell-cycle studies.
  • Intracellular signaling. Phosphorylation analysis
  • Studies of apoptosis with PI, Annexin V and TUNEL
  • Cell proliferation studies via BrdU incorporation or FESS.
  • Detection of intracellular cytokines.
  • Quantification of soluble cytokines by multiplexed assays
  • Cell culture service
  • Maintenance of the general conditions for optimal utilization of the service
  • Contamination detection (mycoplasma)
  • This service will include the preparation and aliquoting of antibiotics specific to culture media, and inactivation and storage of fetal bovine serum, culture media preparation, as well as cell freezing medium and pipette maintenance.
  • Collection and isolation of primary cultures and cell lines.
  • Isolation and obtaining of different cell subpopulations utilizing a magnetic beads system.
  • Cell transformations. Obtain monocyte-derived dendritic cells.
  • Isolation of lymphocytes and marking them for studies of cell proliferation.

Immunochemistry service

  • Technique for quantification of specific antibodies and competitive inhibition assays using radioimmunoassay, fluoroimmunoassay, enzyme immunoassay and microarray system.
  • Electrophoresis (SDS-PAGE) and immunoblotting techniques; Affinity chromatography.

Processing of samples and sterilization of instruments and reagents.

  • Processing of different biological samples: cell separation by density gradient, obtaining of serum and plasma …
  • Sterilization of instruments and reagents.
  • Cristobalina Mayorga Mayorga
  • Fernando Cardona Díaz
  • Fernando Rodríguez de Fonseca