- Methylation and hydroxymethylation analysis

- Scientific-methodological advising on experiment design, choice, and optimization of genomic analysis techniques

- Nucleic acid extraction

- Quantification of nucleic acids (NANODROP 2000, Qubit)

- Amplification and electrophoresis

- Reverse transcription reaction

- Genotyping and genetic expression studies (qPCR, Pyrosequencer)

- Methylation and hydroxymethylation analysis

- Microbiota analysis (16S rRNA)

- Allele quantification (pyrosequencing)

- Duplicated qPCR

- Next-Generation Sequencing (NGS)

- NGS library construction

- ARRAYS (expression, SNPs)

All nodes have refrigerators for reagents and samples, freezers (-20ºC, -80ºC), flow hoods with extraction and UV, centrifuges, end-point thermocyclers, and a nucleic acid quantifier

• IBIMA Plataforma BIONAND Building at the Malaga TechPark
• Fluorimetro Biotek Synergy MX
• Qubit 4
• Nanodrop 2000C
• QIAamplifier 96, Qiagen
• Termociclador Bio-rad
• CFX 96 Real TIme System, Bio-Rad
• MyCycler gradient, Bio-Rad
• QuantStudio 12K Flex bloque arrays
• QuantStudio 12K Flex bloque 384
• PyroMark Q48 Autoprep
• Ion Chef System
• Ion Torrent S5
• Avant Genetic Analyzer 3130XL, Applied Biosystems

Virgen de la Victoria University Hospital Research Laboratory

• QuantStudio 6 (necessary training for their use)

Civil Hospital Research Laboratory

• Maxwell Robot (nucleic acid extraction)

• Robot QiaCube (nucleic acid extraction)

• Qubit Spectrophotometer

• Fluorimeter quantification of nucleic acids (FLx800)

• Thermocyclers

• Mastercycler gradient, Eppendorf

• Veriti 96-Well Thermal Cycler, Applied Biosystems

• The LightCycler® 480 Instrument II, Roche Diagnostics

• 7500 Fast Real-Time PCR System, Thermo Fisher Scientific

• PyroMark Q96 ID, Qiagen