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Electron Microscopy Service

The Electron Microscopy Service offers access to transmission electron microscopy (TEM), cryo-TEM and electron tomography, high resolution scanning electron microscopy (SEM) and environmental SEM. A special emphasis has been made on high-end sample preparation techniques through cryo-inmobilization. All the equipments have been configured to provide its best on biological and nanomedicine applications.

Advanced Optical Imaging Service

Advanced Optical Imaging Service: Confocal and Multiphoton Microscopy

Laser-scanning confocal microscopy is a very popular technique that uses a combination of laser illumination and a “pinhole” mask to ensure that only fluorescence from the focal plane reaches the detector. This avoids the characteristic blur typical of conventional fluorescence microscopy and allows images to captured as detailed optical slices that can be used to build up rich 3D models. Confocal microscopy is one of the most versatile and widely used techniques in optical microscopy. We offer Leica SP5 confocal microscopes featuring HyD hybrid detectors for the best possible sensitivity, as well as full environmental control for live cells and high-speed resonant scanning.

Multi- or two-photon (2P) microscopy takes advantages of the near simultaneous absorption of two or more photons which act to excite a fluorescent molecule with the combined energy of all the photons. In practice, this means that lower energy infrared (IR) light can be used to see fluorescent molecules that are normally excited by high energy ultraviolet and visible wavelength. As IR light is less susceptible to diffusion and absorption, we can visualise fluorescent molecules at greater depths than conventional microscopy. Infrared light also tends to beless damaging to live tissues than UV or blue excitation, making it ideal for timelapse imaging of model organisms or tissue explants.

Advanced Optical Imaging Service: Super-resolution and Conventional Fluorescence Microscopy

Conventional widefield fluorescence microscopy is still the best choice for many applications. The CCD and CMOS-based sensors used for conventional micros-copy are typically much more sensitive that the photomultiplier tubes used in confocal microscopes and flow cytometers. As the camera captures the whole field of view at the same time, it also allows for faster imaging in many cases. Examples where conventional microscopy may be advantageous include the visualisation of individual molecules, receptors or small organisms such as bacteria and yeast. Total Internal Reflection Fluorescence (or TIRF) is a powerful technique which combines the sensitivity of conventional fluorescence with selective illumination to improve the contrast of features very close to the sample coverslip. TIRF is often used for studies related to membrane dynamics, receptor-ligand interactions and vesicular transport. The intrinsic diffraction of light has historically made it difficult to use fluorescence to distinguish structures closer than 200nm apart. Super-resolution microscopy refers to techniques that selectively activate fluorescent molecules to map their position with up to 10 times more accuracy than conventional fluorescent microscopy. The Nikon N- Storm system is capable of localising molecules with a resolution of up to 20nm and is compatible with all most current localisation protocols and fluorophores (including Alexafluor 647 and Atto488) .

Advanced Optical Imaging Service: High Content Screening microscopy

High content screening (HCS) attempts to bridge the gap between microscopy and cytometry, providing both large quantities of statically useful and phenotypically rich data. HCS combines automated microscopy with customisable algorithms able to detect and quantify many different types of phenotypic information in both fixed and living cultured cells. The Perkin Elmer Operetta HCS system combines automated imaging with advanced Harmony HCS software, which contains many common algorithms such as nuclei and cell detection, as well as the ability to automatically detect complex phenotypes through machine learning.



Small Animal Multimodal Imaging Service

In vivo Optical Imaging

Small animal pre-clinical imaging is a vital tool in biomedical research that allows biological processes and potential medicines to be studied in animal models using non- invasive methods. While MRI, and PET/SPECT represent the gold standard for pre- clinical and translational studies.The use of fluorescent and bioluminescent techniques with small animal disease models hasbecome increasingly important over recent years and can provide unmatched specificity and sensitivity with appropriate experimental design

Computed Tomography

Computed Tomography (CT) is based on the reconstruction of 2D X-ray images captured at different angles to generate a detailed 3D view of the subject. CT imaging is typically used to provide vital contextual information for other modalities as well as for specific studies into bone density, contrast agents, implantation and regenerative technologies. Our Albira system uses dedicated beds for rats and mice with incorporated gas anaesthesia. The Albira software suite automates the reconstruction process, while the PMOD software suite enables advanced analyses and the export of data into popular formats such as DICOM.

Magnetic Resonance Imaging

Resonance Imaging (MRI) is a highly versatile technique that can provide not only excellent morphological data, but also functional and physiological information as well. Furthermore, important recent advances in the development of highly sensitive contrast agents using nanotechnology have brought MRI into the molecular imaging arena. Thus, small animal MRI scanners have become a key tool in the development of magnetic nanoparticles for medical diagnosis and therapy.

Nuclear Magnetic Resonance Service

The NMR service is equipped with two High resolution NMR Spectrometers: a 400MHz NMR spectrometer for routine experiments and a higher filed, 600 MHz, spectrometer dedicated NMR studies that are more demanding in terms of sensitivity. This system is also equipped with a high resolution magic angle spinning (HR-MAS) NMR to analyze heterogeneous samples (tissue biopsies or partially insoluble compounds). Applications of NMR spectroscopy to chemistry range from structure elucidation of small molecules to studies of ligand-protein interactions. In biomedicine, the use of NMR spectroscopy has expanded from the classical metabolic studies using enriched stable isotope tracers to the more recent NMR-based metabolomics of biofluids (urine, plasma, CSF). The service also has a TD-NMR analyzer for relaxivity measurements at low magnetic field (1.44 T).

SERVICIOS

Electron Microscopy Service

Equipment

Thermo Scientific-FEI Tecnai G2 20 Twin Transmission Electron Microscope

  • LaB6 filament
  • Tomography software
  • Cryo-transmission system
  • EDS/ EDX (Energy Dispersive X-ray Spectroscopy) elemental analyzer

Thermo Scientific-FEI Quanta 250 FEG Scanning Electron Microscope

  • Field emission gun
  • Environmental-SEM
  • Wet-STEM
  • EDS/ EDX (Energy Dispersive X-ray Spectroscopy) elemental analyzer

Sample preparation equipment

  • Thermo Scientific-FEI Vitrobot Mark IV, for plunge-freezing of water-solution samples
  • Gatan Turbo Pumping Station, for conditioning of the cryo-holder under vacuum conditions
  • GatanCryo Transfer Workstation, for transferring of grids to the cryo-holder under liquid nitrogen-temperature conditions
  • GatanSmartSet Model 900 Cold Stage Controller, digital controller for temperature conditioning of the cryo-holder
  • Leica ultramicrotome UC7, with cryo-ultramicrotomy module FC7, for semithin and ultrathin sections
  • Leica HPM100, for high pressure freezing cryo-fixation of samples at low temperatures
  • Leica AFS2, for freeze substitution resin-embedding of samples at low temperatures, with automatic reagent dispenser FSP
  • Leica SCD500, for metal-thin film deposition on samples for scanning electron microscopy, and glow-discharge of film-coated grids for transmission electron microscopy
  • Leica KMR3, glass-knife maker for ultramicrotomy
  • Leica MP, mounting hot-plate for plastic boats on glass knives

Services Offered

  • Transmission electron microscopy of resin-embedded and non-embedded samples, and elemental analysis using Energy Dispersive X-ray Spectroscopy (EDX/ EDS).
  • Electron Tomography of room temperature (conventional) and frozen-hydrated samples (cryo-electron tomography)
  • Scanning electron microscopy for structural studies of samples and elemental analysis using Energy Dispersive X-ray Spectroscopy (EDX or EDS).
  • Environmental scanning electron microscopy of hydrated samples and dynamic
  • Wet-scanning transmission electron microscopy of samples in thin-film water
  • Resin embedding of samples at room temperature (conventional) of low temperature (freeze-substitution) for
  • Plunge-freezing of samples in water solution (vitrification) for cryo-transmission electron
  • Cryo-fixation of samples at high pressure (High Pressure Freezing).
  • Ultramicrotomy of resin-embedded sections (semithin and ultrathin sections).
  • Glow-discharge of film-coated transmission electron microscopy
  • Data and image
  • Technical advice for users, regarding selection of protocols and procedures for experiments involving electron

Advanced Optical Imaging Service

Advanced Optical Imaging Service: Confocal and Multiphoton Microscopy

Equipment

Leica SP5 HyD Confocal Microscope

  • 2 High Sensitivity HyD and 3 standard PMT spectral
  • High-speed Resonant scanner option (28 frames/sec @ 512 x 512).
  • Objective lenses include 63x (1.4 NA) and 40x (1.3 NA) Plan

9 Laser lines: 405nm, 458nm, 476nm, 488nm, 496nm, 514nm, 561nm, 594nm and 633nm.

  • Microscope enclosure with temperature, humidity and CO2
  • High-precision motorised stage with multi-point and multi-tile software 3 nm resolution galvometric z-axis control.

Leica SP5 HyD MP Multiphoton/Confocal Microscope

  • 2 HyD detectors for multiphoton imaging, 4 PMT spectral
  • Objective lenses include Plan IRAPO 25x (0.95 NA) water immersion 2mm LWD.
  • SpectraphysicsMaiTaiIR pulse laser for multi-photon imaging,tuneable from 700- 1040 nm equivalent to approx. 350-520 nm single photon
  • 8 Laser lines (for confocal imaging): 405nm, 458nm, 476nm, 488nm, 514nm, 561nm, 594nm,
  • Microscope enclosure with temperature, humidity and CO2
  • High-precision motorised stage with multi-point and multi-tile software 3 nm resolution galvometric z-axis control.

Applications

  • 3D imaging of fixed multi-colour immunofluorescence
  • Long-term and high-speed live cell
  • FRAP(Fluorescence Recovery   after   Photobleaching)    and    photoactivation methods for studying molecular
  • Foster Resonance Energy Transfer (FRET) for studying molecular interactions at sub–nanometric
  • Characterisation of single (1P) and two (2P) photon fluorescence properties of novel materials in vitro and in vivo.
  • Two-photon deep tissue imaging (>100 microns) of fluorescent proteins

Services Offered

  • Experimental design advice and consulting for project
  • Integrated cellular interaction analyses; we offer rapid assays analysing uptake and subcellular localisation of fluorescently-labelled molecules in standard cultured cell lines.
  • Quantitative, semi-quantitative and comparative analyses of fluorescent expression/staining in different models. We can advise on the required controls or limitations of different methodologies.
  • Co-localisation studies comparing localisation with standard sub-cellular markers, fluorescent proteins and antibodies with rigorous statistical analysis performed using commercial (IMARIS) and open-source (FIJI) co-localisation analysis tools.
  • We specialise in the long-term (>4d) microscopic visualisation of cell models including primary cells, model organisms and bacterial
  • Sample preparation services include fixation, antibody staining and sample Where required we can troubleshoot and optimise difficult staining combinations.
  • We can supply small aliquots of wide range of standard staining reagents, fluorophores and secondary antibodies and we also offer a custom antibody labelling
  • 3D quantification and visualisation using the advanced IMARIS 3D analysis package including volume quantification, 3D object tracking and cell type and subcellular organelle counting.
  • Two photon emission and excitation spectra measurements and Quantum efficiency (QE) estimationby comparison with reference compounds.
  • High resolution intravital imaging of sub-surface tissues taking advantage of higher tissue penetrate of two-excitation and the long working distance 25x water immersion objective.

Advanced Optical Imaging Service: Super-resolution and Conventional Fluorescence Microscopy

Equipment

 Nikon Eclipse TiTIRF / N-STORM

  • Andor iXon3 897 EM-CCD
  • 100x Plan Apo Oil immersion Objective 49 NA.
  • 4 Excitation lasers405nm, 488nm (200mW), 561nm and 647nm (300mW)
  • TIRF optical
  • Multipass TIRF and STORM filtersfor high-speed multicolour
  • Removable cylindrical lens for 3D
  • Nikon PFS (Perfect Focus System) for improved stability

Nikon Eclipse Ti basic Fluorescence Microscope

  • Inverted Nikon Ti Eclipse fully motorised microscope
  • Hamamatsu Orca R2 monochrome 3 MP CCD camera
  • Objective lenses: 60x (1.4 NA), 40x, 10x and 4x
  • Filter sets for DAPI, GFP, TRITC
  • Configured for both NIS-Elements and the open-source Micro-manager suite for advanced long-term imaging
  • Differential interference contrast (DIC) for improved contrast in brightfield

Nikon / EppendorfMicroinjection System

  • Inverted Nikon Ti Eclipse Microscope
  • Eppendorfmicro-manipulation and injection system
  • Colour fluorescence camera with filters for multi-colour imaging

Nikon /Hamilton ThornePhotoablation System

  • Inverted Nikon Ti Eclipse
  • Hamilton Thorne XYClonephotoablation module

Leica M165 Fluorescent Stereo-microscope

  • High-end 1 x Plan Apo objective lens with 5:1 optical zoom
  • Resolution of up to 1 microns
  • Fluorescence (Hoechst/DAPI, GFP/FITC and TRITC)
  • 8 MP Colour and 3 MP Monochromatic camera options
  • Epi, Trans and Oblique white light illumination

Applications

  • Nanoscale imaging of fixed samples
  • Dynamic imaging of cytoskeletal structures, focal adhesion formationas well as endocytosis and vesicle dynamics in live
  • Single molecule studiesand localisation microscopy modalities including N- STORM, Direct STORM and PALM.
  • Fluorescent analysis of histological
  • Microinjection of DNA, RNA, morpholinos, vital dyes or pharmacological agents into cultured cells, oocytes or
  • Non-contact ablation of targeted membranes or structures as a powerful tool for stem cell, cloning and developmental biology
  • High quality macro-to-micro imaging of bothbiological and non-biological samples

Services Offered

  • Super-resolution (SR) advice and consulting: we can help you evaluate and choose the best localisation microscopy strategies for your
  • We can supply super-resolution specific reagents including buffers and fluorochromes and custom labelled antibodies.
  • STORM image processing: we can convert localisation imaging data into SR images using both proprietary and open source software packages.
  • TIRF imaging of membrane and/or cytoskeletal dynamics in live adherent cells using commercial fluorescent labels such as DiR, FM4-64 and Cell-Light or user-supplied
  • Long term (>4 day) imaging of bacterial colonies and bacterial interactions using DIC, temperature control, autofocus and multi-field
  • Routine multi-colour fluorescent analysis of histological samples where confocal or other more advanced methods are not
  • Imaging of bone, muscle or connective tissues in histological samples using circularly-polarised

Advanced Optical Imaging Service: Super-resolution and Conventional Fluorescence Microscopy

Equipment

 Nikon Eclipse TiTIRF / N-STORM

  • Andor iXon3 897 EM-CCD
  • 100x Plan Apo Oil immersion Objective 49 NA.
  • 4 Excitation lasers405nm, 488nm (200mW), 561nm and 647nm (300mW)
  • TIRF optical
  • Multipass TIRF and STORM filtersfor high-speed multicolour
  • Removable cylindrical lens for 3D
  • Nikon PFS (Perfect Focus System) for improved stability

Nikon Eclipse Ti basic Fluorescence Microscope

  • Inverted Nikon Ti Eclipse fully motorised microscope
  • Hamamatsu Orca R2 monochrome 3 MP CCD camera
  • Objective lenses: 60x (1.4 NA), 40x, 10x and 4x
  • Filter sets for DAPI, GFP, TRITC
  • Configured for both NIS-Elements and the open-source Micro-manager suite for advanced long-term imaging
  • Differential interference contrast (DIC) for improved contrast in brightfield

Nikon / EppendorfMicroinjection System

  • Inverted Nikon Ti Eclipse Microscope
  • Eppendorfmicro-manipulation and injection system
  • Colour fluorescence camera with filters for multi-colour imaging

Nikon /Hamilton ThornePhotoablation System

  • Inverted Nikon Ti Eclipse
  • Hamilton Thorne XYClonephotoablation module

Leica M165 Fluorescent Stereo-microscope

  • High-end 1 x Plan Apo objective lens with 5:1 optical zoom
  • Resolution of up to 1 microns
  • Fluorescence (Hoechst/DAPI, GFP/FITC and TRITC)
  • 8 MP Colour and 3 MP Monochromatic camera options
  • Epi, Trans and Oblique white light illumination

Applications

  • Nanoscale imaging of fixed samples
  • Dynamic imaging of cytoskeletal structures, focal adhesion formationas well as endocytosis and vesicle dynamics in live
  • Single molecule studiesand localisation microscopy modalities including N- STORM, Direct STORM and PALM.
  • Fluorescent analysis of histological
  • Microinjection of DNA, RNA, morpholinos, vital dyes or pharmacological agents into cultured cells, oocytes or
  • Non-contact ablation of targeted membranes or structures as a powerful tool for stem cell, cloning and developmental biology
  • High quality macro-to-micro imaging of bothbiological and non-biological samples

Services Offered

  • Super-resolution (SR) advice and consulting: we can help you evaluate and choose the best localisation microscopy strategies for your
  • We can supply super-resolution specific reagents including buffers and fluorochromes and custom labelled antibodies.
  • STORM image processing: we can convert localisation imaging data into SR images using both proprietary and open source software packages.
  • TIRF imaging of membrane and/or cytoskeletal dynamics in live adherent cells using commercial fluorescent labels such as DiR, FM4-64 and Cell-Light or user-supplied
  • Long term (>4 day) imaging of bacterial colonies and bacterial interactions using DIC, temperature control, autofocus and multi-field
  • Routine multi-colour fluorescent analysis of histological samples where confocal or other more advanced methods are not
  • Imaging of bone, muscle or connective tissues in histological samples using circularly-polarised

Advanced Optical Imaging Service: High Content Screening microscopy

Equipment

Perkin Elmer Operetta HCS

  • 10x, 20x and 40x objective
  • Temperature control and CO2 for cultured
  • Fully automated image acquisition from 24,96 and 384 well
  • Latest version Harmony 6: integrated acquisition and analysis software.
  • Real-time automated analysis and quantification of image
  • Machine learning-based phenotype recognition algorithms (Phenologic)
  • Offline Harmony analysis workstation for improved

Thermofisher Versette liquid handling robot for 96-well plates

  • Programmable robot system
  • 6 position stage: exchange between up to 6 plates or reservoirs
  • Suitable for plate cloning and serial dilutions

Applications

  • Fast and statistically robust toxicity assays using adherent cells with the advantage of being able to visually confirm cell
  • Drug dose response curve calculation: On a single plate multiple concentrations or combinations of compounds can be easily and quickly
  • Screening for specific phenotypes in cultured cells using libraries of small molecules, siRNAs or other biologically-active
  • Screening for complex phenotypes: many cytological effects cannot be detected easily by Operetta allows complex phenotypes (e.g. organelle distribution or cell organisation changes) to be detected automatically and reliably.
  • Long term cell culture studies: Operetta is equipped with temperature and CO2 control making it suitable for maintaining live cultured cells in optimal conditions during image

Services Offered

  • High content screening design and setup advice. The most important step in performing a successful HCS screen is good experimental design and
  • Experimental validation and pilot study testing. For more complex projects it is essential to perform preliminary tests to identify possible problems with labelling, marker expression, cell morphology and other sources of variability before expending valuable time and
  • Custom experiments and HCS analysis design: we can design both assays and custom automatic image analysis pipelines using the Harmony platform based on your
  • Integrated cytotoxicity testing service. We have developed robust protocols from the incubation of cultured cells with test compounds to measuring cytotoxic responses at different time points. Different criteria such as plasma membrane integrity, metabolic activity or intracellular esterase activity can be chosen depending on experimental
  • Development of custom liquid handing Using the Thermofisher Versette liquid handing system we can develop tailor-made solutions to facilitate medium-to-large scale experiments in 96 well plates with greater accuracy and lower overall costs.

Small Animal Multimodal Imaging Service

In vivo Optical Imaging

Equipment

 Bruker In-Vivo Xtreme Multimodal Imaging System

  • High sensitivity CCD camera suitable for
  • Illumination and filters compatible with a wide range of visible and NIR fluorescence markers (Ex. 410 – 760 nm / Em430 – 850 nm)
  • Imaging chamber for up to five mice at a time with integrated anaesthesia and temperature
  • Integrated high resolution X-ray imaging to provide contextual
  • Multimodal Animal Rotation System (MARS) – enables automated image acquisition at different anglesreducing positional variability and aiding the quantification of signal
  • Multimodal Animal Transport System (MATS) – facilitates multimodal imaging between different imaging platforms

Applications

  • Bioimaging of labelled cells/tissues using fluorescence or
  • Monitoring of Drug Delivery and
  • Pharmacodynamic
  • Imaging and validating new Probes and

Services Offered

  • Experimental design advice and project plan
  • Phantom-based experimental assessment of labelling
  • Real time bioimaging of labelled cells or pharmacological agents during and immediately after intravenous
  • Medium-term (1-14 days) biodistribution studies of fluorescently-labelled agents over days or weeks with ex-vivo
  • Design custom spectral unmixing profiles to reduce autofluorescence or crosstalk between overlapping markers.
  • Establishment  of   disease models using commercial molecular imaging
  • Close integration with other BIONAND units for associated animal handling and histology

Computed Tomography

Equipment

  • Albira small animal CT system
  • Dedicated beds for individual rats and mice with
  • MATS bed for simultaneous imaging of up to four mice with anaesthesia
  • Albira Software Suite 0 with integrated acquisition and tomography.
  • PMOD biomedical image quantification
  • 90 µM resolution and 70 mm diameter Field of View (FOV).
  • Energy Range (kVp) 10-50.
  • QRM Micro-CT HA Phantom 0, 50, 200, 800,1200 mg HA/cm3

Applications

  • Dentistry and bone regeneration
  • Contextual / anatomical imaging in conjunction with other
  • Bone density
  • Implantation studies

Services Offered

  • Phantom-based measurement of reagent densities (e.g. as part of the characterisation of X-ray/CT-based contrast agents)
  • Bone-density measurements     based     on     calibration     using     a     calcium hydroxyapatite phantom.
  • Image processing, analysis and quantification services including specialised packages such as PMOD, FIJI and BoneJ.
  • Advanced custom 3D visualisations using the Imaris software
  • Close integration with other BIONAND units for associated animal handling and histology

Magnetic Resonance Imaging

Equipment

  • Bruker BioSpec 4T/20 animal MRI system
  • Magnetic field strength of 4T
  • 20 cm bore diameter, suitable for mice and rats
  • Automated positioning system (AutoPac™)
  • High performance actively shielded gradient system (400 mT m-1) with integrated shims
  • RF coils for rats and mice

Applications

  • Anatomical Studies: tumors, inflammation, degenerative diseases,
  • Functional Studies: brain perfusion, vascular permeability, in vivo metabolic profiling, cardiac imaging,
  • Pharmacodynamics and Biodistribution of magnetic Contrast Agents (or any drug magnetically labeled)
  • Monitoring of Drug Delivery and
  • Follow up of Therapy Response

Services Offered

  • Experimental design advice and project plan
  • Morphological imaging: T1-weighted, T2-weighted, PD, diffusion-weighted,
  • T1 and T2 Dynamic
  • Diffusion Imaging: ADC,
  • Phantom-based relaxivity
  • Cardiac
  • Single Voxel
  • Image processing, analysis and
  • Close integration with other BIONAND units for associated animal handling and histology

Nuclear Magnetic Resonance Service

Equipment

Bruker Ascend 400 MHz NMR spectrometer

  • Ultra ShieldTM Plus 4T magnet
  • 5 mm BBFO PLUS probe, optimized for X-nuclei direct Broad band in a frequency range between 31P and 15N

Bruker Avance 600 MHz NMR spectrometer

  • Ultra ShieldTM Plus 1T magnet
  • 5 mm BBFOPLUS probe, optimized for X-nuclei direct observation in a frequency range between 31P and 15N
  • 5 mm TXI probe, optimized for higher sensitivity 1H-nuclei observation
  • TCI CryoProbe Prodigy, uses nitrogen-cooled RF coils and preamplifiers for 1H, 2H and 13C, delivering a 5x 1H sensitivity boost
  • HR-MAS Probe

Bruker Minispec (TD-NMR) analyzer

  • Permanent 4T magnetic field
  • Intelligent 1H probe (plug and play) with 60 MHz operating frequency

Applications

  • Structure verification and elucidation
  • Molecular mobility: determination of conformation in solution
  • Determination of enantiomeric excess
  • Kinetic and temperature studies of reaction mixtures
  • Metabolomic analysis of biofluids (urine, blood serum, )
  • Metabolomic analysis of intact tissue (biopsies)
  • Relaxivity measurement    of    magnetic    compounds,    such    as    magnetic nanoparticles or compounds containing lanthanides (Gd, Dy, )

Services Offered

  • Experimental design advice and project plan
  • Acquisition of 1D NMR spectra at 400 and 600 MHz (1H, 13C, 31P, etc.)
  • Acquisition of basic 2D experiments: COSY, TOCSY, HSQC, HMBC, J-RES
  • Nuclear Overhauser Effect experiments (NOE and ROE), Diffusion experiments (DOSY), selective excitation experiments.
  • Acquisition of 1H and 31P HR-MAS NMR
  • Preparation of biofluid samples (urine, blood serum/plasma) for high resolution NMR spectroscopy following standardized
  • Preparation of tissue samples for HR-MAS NMR
  • Basic processing of NMR
  • Relaxivity measurements (r1 and r2) at low magnetic field (1.44 T).

CONTACTS

Scientific Manager

Technical staff

Preclinical image

Resonance

DOCUMENTS

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Calle Severo Ochoa, 35
Parque Tecnolgico de Andaluca (PTA) Campanillas, Mlaga 29590

(+34) 951 440 260
Fax: (+34) 951 440 263